Single-molecule Sequencing

DNA sequencing was invented at the LMB by Fred Sanger in the late 1970s and, for many applications, Sanger sequencing remains the gold standard due to its accuracy and relatively long (~1kb) reads.

Many new sequencing technologies have been developed since then, some of them capable of producing billions of bases of sequence in a single run. However, most of these methods give short reads, limiting their utility.

For very long (10kb-100kb) reads, single molecule methods are essential. Any method which operates on multiple molecules runs into the problem of synchronization: the 'output' from the different molecules eventually drifts out of synch, smearing out the data to the point of unreadability.

I have been working for some time on a single molecule sequencing method, with the aim of getting extremely long reads from native genomic DNA.

This work is now the subject of commercial development, and more details will be posted at a later date.